This second volume provides new and up-to-date methods of the intestinal stem cell field. Chapters guide readers through in vitro techniques, single-cell transcriptional profiling method, the isolation of intestinal crypts to generate, establish 3D organoids, animal models of gastrointestinal cancer, and examples of the use of in vivo methods for studying intestinal tumor-initiating cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and state-of-the-art,Intestinal Stem Cells: Methods and Protocols Second Edition, aims to provide comprehensive and easy to follow protocols.