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Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers (Methods in Molecular Biology, 605) (en Inglés)
Weissig Volkmar (Autor)
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Humana
· Tapa Blanda
Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers (Methods in Molecular Biology, 605) (en Inglés) - Weissig Volkmar
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Reseña del libro "Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers (Methods in Molecular Biology, 605) (en Inglés)"
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that "the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer" [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
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